Immobilization of Bacillus sp. Strain Neni-8 in Dialysis Tubing Reduced Copper Toxicity to the Molybdenum Reduction Process

In ruminants, even trace amounts of molybdenum can be lethal. areas with high pollution, levels in soil and mine tailings exceed 20,000 ppm. Bioremediation challenging when toxic copper is also present. This research presents a novel approach using dialysis tubing the molybdenum-reducing activity Bacillus sp. strain Neni-8 for removal from aqueous solutions. Molybdenum blue (Mo-blue), produced during enzymatic reduction, insoluble this twofold advantage as method to protect bacterial cells heavy metal inhibition, especially copper. experiment, we assess toxicity-shielding effect reduction Mo-blue by bacterium presence As concentrations were increased, both free immobilized strongly inhibited. Modelling dissociationone-phase exponential decay model gave an IC50 value form 0.1107 mg/L (95% confidence interval 0.073 0.217 while cell system was 0.023 C.I. 0.019 0.028). Since values did not overlap, better protection than system. Toxicity higher toxicity trapped tubes, suggesting that trapping Mo-reducing may effective strategy bioremediation water or wastewater contaminated multiple metals.